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QIAGEN ROTOR GENE 6000 SOFTWARE SOFTWARE

HRM characterizes double-stranded PCR products based on their dissociation (melting) behavior. High-resolution melting analysis (HRM) is a closed-tube, post-PCR analysis that has raised enormous scientific interest. JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow

QIAGEN ROTOR GENE 6000 SOFTWARE SOFTWARE

In addition, the software allows you to create new excitation/detection wavelength combinations, which means that the Rotor-Gene Q is compatible with dyes you may use in the future.

With up to 6 channels spanning UV to infrared wavelengths, the cycler delivers the widest optical range currently available (see table). Whether your assay is based on intercalating dyes such as SYBR ® Green, probes such as hydrolysis (TaqMan®), hybridization (FRET), Scorpion probes, or other multiplex chemistries, the Rotor-Gene Q meets your requirements. Unrivaled optical range enables multiple applications

Fast ramping and negligible equilibration times for short run-times.

Uniform detection eliminating the need for ROX reference dye.

These are unavoidable in traditional block-based instruments due to temperature gradients across the block and multiple, complex optical pathways. It also eliminates sample-to-sample variations and edge effects. This thermal and optical uniformity results in sensitive, precise, and fast real-time PCR analysis (see figure " Precise real-time PCR analysis").

When each tube aligns with the detection optics, the sample is illuminated and the fluorescent signal is rapidly collected from a single, short optical pathway. Each tube spins in a chamber of moving air, keeping all samples at precisely the same temperature during rapid thermal cycling. The unique centrifugal rotary design of the Rotor-Gene Q makes it the most precise and versatile real-time PCR cycler currently available (see figure " Cross-section of the Rotor-Gene Q"). Unique rotary design for outstanding performance